Gru is no longer a villain after saving his three adopted daughters Margo, Edith, and Agnes from Vector in the first film Despicable Me. His calm and unexciting new life takes an unexpected turn when he is recruited by Lucy Wilde and Silas Ramsbottom and is taken to the headquarters of the Anti-Villain League, a society dedicated to fighting crime on a global scale. There, he discovers that there is a new villain named El Macho on the loose. Because Gru was once a villain himself and knows how they think, Silas (the League’s head), wants Gru to help them fight the villain. With the help of Gru’s assistant, Dr. Nefario, his minions, Margo, Edith, and Agnes, Gru must save the world (“again”).